Detailed information of TA system TA126956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 90563..91214
• Replicon: chromosome
• Accession: NZ_CP040780
• Organism: Lacticaseibacillus rhamnosus strain 1.0320

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: FG342_RS00485
• Protein ID: WP_005686631.1
• Coordinates: 90563..90946 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: FG342_RS00490
• Protein ID: WP_005686632.1
• Coordinates: 90966..91214 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FG342_RS00470	89522..90049	-	528	WP_047678030.1	QueT transporter family protein	-
FG342_RS14935	90184..90339	+	156	WP_081262725.1	alpha-galactosidase	-
FG342_RS00485	90563..90946	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FG342_RS00490	90966..91214	-	249	WP_005686632.1	antitoxin	Antitoxin
FG342_RS00495	91326..92465	-	1140	WP_047677957.1	alanine racemase	-
FG342_RS00500	92452..92826	-	375	WP_005686634.1	holo-ACP synthase	-
FG342_RS00505	92995..94503	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
FG342_RS00510	94777..96165	-	1389	WP_047677959.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T126956 WP_005686631.1 NZ_CP040780:c90946-90563 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T126956 NZ_CP040780:c90946-90563 [Lacticaseibacillus rhamnosus]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAACAATGTTGGTAACCATTATAGCCCCACTGTTATCGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGCAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGCTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
GGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCAAAGCCGAAAACATATAATAAAAACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT126956 WP_005686632.1 NZ_CP040780:c91214-90966 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT126956 NZ_CP040780:c91214-90966 [Lacticaseibacillus rhamnosus]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGCGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19