Detailed information of TA system TA126938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 126183..126702
• Replicon: plasmid pVRE1-VanA
• Accession: NZ_CP040742
• Organism: Enterococcus faecium strain VRE1

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: FGY81_RS15970
• Protein ID: WP_000588503.1
• Coordinates: 126445..126702 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: FGY81_RS15965
• Protein ID: WP_000388479.1
• Coordinates: 126183..126452 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGY81_RS15925	121802..122206	-	405	WP_002287522.1	IS200/IS605-like element ISEfa4 family transposase	-
FGY81_RS15930	122539..122955	-	417	WP_002305824.1	hypothetical protein	-
FGY81_RS15935	122957..123238	-	282	WP_002347613.1	hypothetical protein	-
FGY81_RS15940	123614..124138	-	525	WP_044383356.1	DUF536 domain-containing protein	-
FGY81_RS15945	124232..124912	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
FGY81_RS15950	124974..125492	+	519	WP_175415673.1	hypothetical protein	-
FGY81_RS15960	125873..126052	+	180	WP_001120991.1	hypothetical protein	-
FGY81_RS15965	126183..126452	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FGY81_RS15970	126445..126702	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
FGY81_RS15975	127161..128165	+	1005	WP_000455809.1	hypothetical protein	-
FGY81_RS15980	128446..129126	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
FGY81_RS15985	129181..129615	+	435	Protein_161	JAB domain-containing protein	-
FGY81_RS15990	129641..130186	+	546	WP_002320753.1	hypothetical protein	-
FGY81_RS15995	130187..130753	+	567	WP_002320752.1	hypothetical protein	-
FGY81_RS16000	130768..131016	+	249	WP_002350598.1	hypothetical protein	-
FGY81_RS16005	131036..131275	+	240	WP_002320750.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / ant(6)-Ia / aph(3')-III	-	1..132733	132733
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III	-	94230..129126	34896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T126938 WP_000588503.1 NZ_CP040742:126445-126702 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T126938 NZ_CP040742:126445-126702 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT126938 WP_000388479.1 NZ_CP040742:126183-126452 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT126938 NZ_CP040742:126183-126452 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7