Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Xre-MNT/HTH_26(antitoxin)
Location 108787..109895 Replicon plasmid pVRE1-VanA
Accession NZ_CP040742
Organism Enterococcus faecium strain VRE1

Toxin (Protein)


Gene name MNTss Uniprot ID A0A0M4MHJ8
Locus tag FGY81_RS15845 Protein ID WP_002303392.1
Coordinates 109026..109895 (+) Length 290 a.a.

Antitoxin (Protein)


Gene name Xress Uniprot ID B9DSK1
Locus tag FGY81_RS15840 Protein ID WP_002303393.1
Coordinates 108787..109011 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGY81_RS15815 105246..105542 + 297 WP_138821096.1 type III secretion system protein PrgN -
FGY81_RS15820 105566..106525 - 960 WP_002287225.1 IS30 family transposase -
FGY81_RS15825 106708..107307 + 600 Protein_132 hypothetical protein -
FGY81_RS15830 107383..108063 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
FGY81_RS15835 108339..108644 + 306 WP_099097609.1 site-specific recombinase -
FGY81_RS15840 108787..109011 + 225 WP_002303393.1 helix-turn-helix transcriptional regulator Antitoxin
FGY81_RS15845 109026..109895 + 870 WP_002303392.1 nucleotidyltransferase domain-containing protein Toxin
FGY81_RS15850 109876..110610 + 735 WP_000662263.1 class I SAM-dependent methyltransferase -
FGY81_RS15855 110643..111551 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
FGY81_RS15860 111548..111796 + 249 WP_002347175.1 hypothetical protein -
FGY81_RS15865 111799..112707 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
FGY81_RS15870 112808..114103 - 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
FGY81_RS15875 114348..114599 + 252 Protein_142 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / ant(6)-Ia / aph(3')-III - 1..132733 132733
- inside IScluster/Tn ant(6)-Ia / aph(3')-III - 94230..129126 34896


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polymerase, nucleotidyl transferase domain

(12-111)

Antitoxin

Cro/C1-type helix-turn-helix domain

(4-57)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32807.38 Da        Isoelectric Point: 4.7269

>T126937 WP_002303392.1 NZ_CP040742:109026-109895 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIYYNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Download         Length: 870 bp

>T126937 NZ_CP040742:109026-109895 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATTGTATTAGGGGGCTC
ACGTGCAAGAAGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAGTTGGATGATGAGAATAGAAACAACCTTGTTGTACCTCCCGGAGCGTGGGGTGATTGG
GTTAATGGCGGTGGATGGTTAGTTATAAACGGGTGTCATGTTGACTTGATATTACGTGATATAAAACGGGTGGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCTATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGCAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGACATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8351.65 Da        Isoelectric Point: 4.5357

>AT126937 WP_002303393.1 NZ_CP040742:108787-109011 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGDEIKISNE

Download         Length: 225 bp

>AT126937 NZ_CP040742:108787-109011 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTTAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGATGAAATAAAAATATCTAACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MHJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M5SHM7

References