Detailed information of TA system TA126935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 52151..52758
• Replicon: plasmid pVRE1-1
• Accession: NZ_CP040741
• Organism: Enterococcus faecium strain VRE1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: FGY81_RS14040
• Protein ID: WP_002321032.1
• Coordinates: 52408..52758 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: FGY81_RS14035
• Protein ID: WP_002287514.1
• Coordinates: 52151..52414 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGY81_RS13990	47608..47868	+	261	WP_002296240.1	hypothetical protein	-
FGY81_RS14000	48309..48515	+	207	WP_002296239.1	hypothetical protein	-
FGY81_RS14005	48515..48766	+	252	WP_002296238.1	hypothetical protein	-
FGY81_RS14010	48781..49218	+	438	WP_002296237.1	hypothetical protein	-
FGY81_RS14015	49211..49918	+	708	WP_002353391.1	hypothetical protein	-
FGY81_RS14025	50299..50478	+	180	WP_002305761.1	hypothetical protein	-
FGY81_RS14030	50786..51958	-	1173	WP_002323245.1	IS256-like element IS1542 family transposase	-
FGY81_RS14035	52151..52414	+	264	WP_002287514.1	hypothetical protein	Antitoxin
FGY81_RS14040	52408..52758	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FGY81_RS14045	52782..53386	-	605	Protein_54	recombinase family protein	-
FGY81_RS14050	53786..54958	+	1173	WP_002323245.1	IS256-like element IS1542 family transposase	-
FGY81_RS14055	55032..55454	+	423	WP_002303116.1	hypothetical protein	-
FGY81_RS14060	55464..55667	+	204	WP_002299573.1	hypothetical protein	-
FGY81_RS14065	55878..56462	+	585	WP_002299575.1	recombinase family protein	-
FGY81_RS14070	56524..57204	-	681	WP_010861579.1	IS6-like element IS1216 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..238664	238664
	-	inside	IScluster/Tn	-	-	50786..75489	24703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T126935 WP_002321032.1 NZ_CP040741:52408-52758 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T126935 NZ_CP040741:52408-52758 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT126935 WP_002287514.1 NZ_CP040741:52151-52414 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT126935 NZ_CP040741:52151-52414 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0