Detailed information of TA system TA126930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 4492..5093
• Replicon: plasmid p1
• Accession: NZ_CP040737
• Organism: Companilactobacillus futsaii strain Y97

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: FG051_RS12780
• Protein ID: WP_056972590.1
• Coordinates: 4749..5093 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: FG051_RS12775
• Protein ID: WP_001748109.1
• Coordinates: 4492..4755 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FG051_RS12750	236..907	+	672	WP_057813087.1	TetR/AcrR family transcriptional regulator	-
FG051_RS12755	1011..1634	-	624	Protein_2	nicotinate phosphoribosyltransferase	-
FG051_RS12760	1636..1890	-	255	Protein_3	DUF536 domain-containing protein	-
FG051_RS12765	1883..2818	-	936	WP_057813089.1	replication initiation protein	-
FG051_RS12770	3819..4406	-	588	WP_149029903.1	site-specific integrase	-
FG051_RS12775	4492..4755	+	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FG051_RS12780	4749..5093	+	345	WP_056972590.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FG051_RS12785	5663..6343	-	681	WP_046811108.1	ABC transporter ATP-binding protein	-
FG051_RS12790	6348..7400	-	1053	WP_046811107.1	ABC transporter permease	-
FG051_RS12795	7526..7747	+	222	WP_046811106.1	transposase	-
FG051_RS12800	8207..8485	+	279	WP_003625288.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FG051_RS12805	8475..8777	+	303	WP_005917927.1	type II toxin-antitoxin system YafQ family toxin	-
FG051_RS12810	9579..9959	+	381	WP_057813085.1	DUF4430 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37880	37880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13111.00 Da
• Isoelectric Point: 6.8698

>T126930 WP_056972590.1 NZ_CP040737:4749-5093 [Companilactobacillus futsaii]
MVSQGDIFYVNFNPRRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T126930 NZ_CP040737:4749-5093 [Companilactobacillus futsaii]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGGCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT126930 WP_001748109.1 NZ_CP040737:4492-4755 [Companilactobacillus futsaii]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT126930 NZ_CP040737:4492-4755 [Companilactobacillus futsaii]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTGACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAAGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A806J926