Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 3729..4372 Replicon plasmid pKpvST147B_NDM-1
Accession NZ_CP040728
Organism Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag FGF03_RS29005 Protein ID WP_000754566.1
Coordinates 3729..4145 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FGF03_RS29010 Protein ID WP_001261276.1
Coordinates 4142..4372 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF03_RS28980 229..564 + 336 WP_011977766.1 C2H2-type zinc finger protein -
FGF03_RS28985 1190..1303 + 114 Protein_1 Tn3 family transposase -
FGF03_RS28990 1311..2387 - 1077 WP_000227969.1 IS110 family transposase -
FGF03_RS29000 2669..3525 - 857 Protein_3 IS3-like element ISEc15 family transposase -
FGF03_RS29005 3729..4145 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
FGF03_RS29010 4142..4372 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FGF03_RS29020 5037..5243 + 207 WP_004153649.1 hypothetical protein -
FGF03_RS29025 5289..5597 + 309 WP_004152336.1 hypothetical protein -
FGF03_RS29030 5625..5954 + 330 WP_004152337.1 hypothetical protein -
FGF03_RS29035 5980..6378 + 399 WP_004171440.1 hypothetical protein -
FGF03_RS29040 6385..6717 + 333 WP_004152339.1 hypothetical protein -
FGF03_RS29045 6717..7499 + 783 WP_004152340.1 site-specific integrase -
FGF03_RS29055 8713..9186 - 474 WP_004152341.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 9306..10574 1268
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaCTX-M-15 / qnrS1 / aph(3')-VI / blaNDM-1 - 1..53955 53955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T126924 WP_000754566.1 NZ_CP040728:c4145-3729 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T126924 NZ_CP040728:c4145-3729 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT126924 WP_001261276.1 NZ_CP040728:c4372-4142 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT126924 NZ_CP040728:c4372-4142 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References