Detailed information of TA system TA126923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 294845..295755
• Replicon: plasmid pKpvST147B_virulence
• Accession: NZ_CP040726
• Organism: Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: FGF03_RS28680
• Protein ID: WP_004026354.1
• Coordinates: 294845..295315 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: FGF03_RS28685
• Protein ID: WP_004181895.1
• Coordinates: 295312..295755 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGF03_RS28655	291443..291820	+	378	WP_029884711.1	hypothetical protein	-
FGF03_RS31255	292018..292992	-	975	WP_058842863.1	hypothetical protein	-
FGF03_RS28665	293595..293753	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
FGF03_RS28675	294475..294735	+	261	WP_004026352.1	hypothetical protein	-
FGF03_RS28680	294845..295315	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
FGF03_RS28685	295312..295755	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
FGF03_RS28690	295856..297127	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
FGF03_RS28695	297127..297549	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
FGF03_RS28705	297635..299122	-	1488	WP_162185051.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / sul2 / armA / msr(E) / mph(E) / dfrA5 / qacE / sul1 / mph(A)	rmpA2 / iutA / iucD / iucC / iucB / iucA / iucA / rmpA	1..339117	339117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T126923 WP_004026354.1 NZ_CP040726:c295315-294845 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T126923 NZ_CP040726:c295315-294845 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT126923 WP_004181895.1 NZ_CP040726:c295755-295312 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT126923 NZ_CP040726:c295755-295312 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9