Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 18098..18690 Replicon plasmid pKpvST147B_3
Accession NZ_CP040725
Organism Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag FGF03_RS26930 Protein ID WP_000520549.1
Coordinates 18098..18346 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag FGF03_RS26935 Protein ID WP_000681613.1
Coordinates 18343..18690 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF03_RS26895 14973..15488 + 516 WP_001025395.1 J domain-containing protein -
FGF03_RS26900 15593..16135 + 543 WP_001083906.1 hypothetical protein -
FGF03_RS26905 16132..16383 + 252 WP_001230707.1 hypothetical protein -
FGF03_RS26910 16370..16597 + 228 WP_000801440.1 hypothetical protein -
FGF03_RS26920 17053..17352 + 300 WP_000650309.1 hypothetical protein -
FGF03_RS26925 17443..17793 + 351 WP_000854259.1 hypothetical protein -
FGF03_RS26930 18098..18346 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
FGF03_RS26935 18343..18690 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FGF03_RS26945 19051..19554 + 504 WP_000781813.1 hypothetical protein -
FGF03_RS26950 19558..20787 + 1230 WP_113439821.1 relaxase/mobilization nuclease domain-containing protein -
FGF03_RS26955 20864..21157 + 294 WP_000134733.1 LuxR family transcriptional regulator -
FGF03_RS26960 21154..21369 + 216 WP_000760375.1 EexN family lipoprotein -
FGF03_RS26965 21727..22347 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
FGF03_RS26970 22415..22687 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31607 31607


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T126921 WP_000520549.1 NZ_CP040725:18098-18346 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T126921 NZ_CP040725:18098-18346 [Klebsiella pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT126921 WP_000681613.1 NZ_CP040725:18343-18690 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT126921 NZ_CP040725:18343-18690 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References