Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2981855..2982480 Replicon chromosome
Accession NZ_CP040724
Organism Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FGF03_RS14905 Protein ID WP_002882817.1
Coordinates 2982097..2982480 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FGF03_RS14900 Protein ID WP_004150355.1
Coordinates 2981855..2982097 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF03_RS14875 2977846..2978445 + 600 WP_004151865.1 glucose-1-phosphatase -
FGF03_RS14880 2978439..2979299 + 861 WP_004146232.1 virulence factor BrkB family protein -
FGF03_RS14885 2979296..2979733 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
FGF03_RS14890 2979778..2980719 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FGF03_RS14895 2980733..2981650 - 918 WP_021466676.1 alpha/beta hydrolase -
FGF03_RS14900 2981855..2982097 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
FGF03_RS14905 2982097..2982480 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FGF03_RS14910 2982654..2983583 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FGF03_RS14915 2983580..2984215 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FGF03_RS14920 2984212..2985114 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T126915 WP_002882817.1 NZ_CP040724:2982097-2982480 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T126915 NZ_CP040724:2982097-2982480 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT126915 WP_004150355.1 NZ_CP040724:2981855-2982097 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT126915 NZ_CP040724:2981855-2982097 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References