Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 1440442..1441085 Replicon chromosome
Accession NZ_CP040724
Organism Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)


Gene name vagD Uniprot ID A0A4S8C081
Locus tag FGF03_RS07215 Protein ID WP_032433387.1
Coordinates 1440442..1440858 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag FGF03_RS07220 Protein ID WP_001261276.1
Coordinates 1440855..1441085 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF03_RS07185 1435611..1435814 - 204 WP_032433376.1 hemolysin expression modulator Hha -
FGF03_RS07190 1435874..1436365 - 492 WP_032433378.1 hypothetical protein -
FGF03_RS07195 1436396..1436914 - 519 WP_045326794.1 hypothetical protein -
FGF03_RS07200 1436973..1437221 - 249 WP_032433382.1 hypothetical protein -
FGF03_RS07205 1437540..1439111 + 1572 WP_032433383.1 ATP-binding protein -
FGF03_RS07210 1439306..1440286 - 981 WP_032433385.1 hypothetical protein -
FGF03_RS07215 1440442..1440858 - 417 WP_032433387.1 type II toxin-antitoxin system VapC family toxin Toxin
FGF03_RS07220 1440855..1441085 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
FGF03_RS07230 1441390..1442205 + 816 WP_032433388.1 hypothetical protein -
FGF03_RS07235 1442733..1444973 - 2241 WP_045326788.1 NTPase -
FGF03_RS07240 1444976..1446063 - 1088 Protein_1407 transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14974.43 Da        Isoelectric Point: 7.8921

>T126911 WP_032433387.1 NZ_CP040724:c1440858-1440442 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALHLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLTLEDWVI

Download         Length: 417 bp

>T126911 NZ_CP040724:c1440858-1440442 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATTATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT126911 WP_001261276.1 NZ_CP040724:c1441085-1440855 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT126911 NZ_CP040724:c1441085-1440855 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAACGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S8C081

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References