Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1423658..1424394 Replicon chromosome
Accession NZ_CP040724
Organism Klebsiella pneumoniae strain KpvST147B_SE1_1_NDM

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag FGF03_RS07115 Protein ID WP_032433360.1
Coordinates 1423658..1424140 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag FGF03_RS07120 Protein ID WP_003026799.1
Coordinates 1424128..1424394 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF03_RS07090 1418670..1419248 + 579 WP_032432061.1 type 1 fimbrial protein -
FGF03_RS07095 1419260..1420204 + 945 WP_077254249.1 fimbrial protein -
FGF03_RS07100 1420417..1421016 + 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
FGF03_RS07105 1421396..1422027 + 632 Protein_1382 hypothetical protein -
FGF03_RS07110 1422056..1423458 - 1403 Protein_1383 ISNCY-like element ISKpn21 family transposase -
FGF03_RS07115 1423658..1424140 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
FGF03_RS07120 1424128..1424394 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
FGF03_RS07125 1424707..1425270 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
FGF03_RS07130 1425280..1425912 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
FGF03_RS07135 1425930..1426934 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
FGF03_RS07140 1426918..1427580 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
FGF03_RS07145 1427609..1428748 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T126910 WP_032433360.1 NZ_CP040724:c1424140-1423658 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T126910 NZ_CP040724:c1424140-1423658 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT126910 WP_003026799.1 NZ_CP040724:c1424394-1424128 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT126910 NZ_CP040724:c1424394-1424128 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References