Detailed information of TA system TA126894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1018..1492
• Replicon: plasmid p16HN-35_5
• Accession: NZ_CP040716
• Organism: Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: FGL96_RS29110
• Protein ID: WP_004197762.1
• Coordinates: 1226..1492 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: FGL96_RS29105
• Protein ID: WP_000879771.1
• Coordinates: 1018..1242 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL96_RS29100 (FGL96_29485)	139..351	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
FGL96_RS29105 (FGL96_29490)	1018..1242	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
FGL96_RS29110 (FGL96_29495)	1226..1492	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGL96_RS29115 (FGL96_29500)	1562..1912	-	351	WP_004197756.1	hypothetical protein	-
FGL96_RS29120 (FGL96_29505)	1978..2523	-	546	WP_023317533.1	hypothetical protein	-
FGL96_RS29125 (FGL96_29510)	2549..2875	-	327	WP_050516389.1	hypothetical protein	-
FGL96_RS29130	4031..4207	+	177	WP_004197757.1	hypothetical protein	-
FGL96_RS29135 (FGL96_29520)	4197..4541	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
FGL96_RS29140	4723..4968	-	246	WP_032433971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T126894 WP_004197762.1 NZ_CP040716:1226-1492 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T126894 NZ_CP040716:1226-1492 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT126894 WP_000879771.1 NZ_CP040716:1018-1242 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT126894 NZ_CP040716:1018-1242 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5