Detailed information of TA system TA126893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1704..2284
• Replicon: plasmid p16HN-35_4
• Accession: NZ_CP040715
• Organism: Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: FGL96_RS29045
• Protein ID: WP_071177730.1
• Coordinates: 1970..2284 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: FGL96_RS29040
• Protein ID: WP_000093040.1
• Coordinates: 1704..1982 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGL96_RS29025 (FGL96_29400)	277..522	+	246	WP_032440458.1	hypothetical protein	-
FGL96_RS29030 (FGL96_29405)	796..1167	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
FGL96_RS29035	1164..1529	+	366	WP_072354022.1	TonB family protein	-
FGL96_RS29040 (FGL96_29415)	1704..1982	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
FGL96_RS29045 (FGL96_29420)	1970..2284	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FGL96_RS29050 (FGL96_29425)	2448..2876	+	429	WP_001140599.1	hypothetical protein	-
FGL96_RS29055 (FGL96_29430)	2902..3081	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
FGL96_RS29060 (FGL96_29435)	3108..3638	-	531	WP_071177729.1	hypothetical protein	-
FGL96_RS29065 (FGL96_29440)	3645..4376	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
FGL96_RS29070 (FGL96_29445)	4376..6340	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T126893 WP_071177730.1 NZ_CP040715:1970-2284 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T126893 NZ_CP040715:1970-2284 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT126893 WP_000093040.1 NZ_CP040715:1704-1982 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT126893 NZ_CP040715:1704-1982 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1