126888

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59551..60076 Replicon plasmid p16HN-35_Vir
Accession NZ_CP040712
Organism Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FGL96_RS27345 Protein ID WP_001159871.1
Coordinates 59551..59856 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FGL96_RS27350 Protein ID WP_000813634.1
Coordinates 59858..60076 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL96_RS27310 (54978) 54978..55775 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
FGL96_RS27315 (55837) 55837..56088 + 252 Protein_74 DUF4113 domain-containing protein -
FGL96_RS27320 (56093) 56093..56485 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
FGL96_RS27325 (56490) 56490..57461 - 972 WP_001103694.1 plasmid segregation protein ParM -
FGL96_RS27330 (57690) 57690..58334 + 645 WP_000633911.1 AAA family ATPase -
FGL96_RS27335 (58328) 58328..58603 + 276 WP_000239529.1 hypothetical protein -
FGL96_RS27340 (58741) 58741..59550 - 810 WP_000016979.1 site-specific integrase -
FGL96_RS27345 (59551) 59551..59856 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FGL96_RS27350 (59858) 59858..60076 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FGL96_RS27355 (60672) 60672..61646 - 975 WP_004213565.1 sensor domain-containing diguanylate cyclase -
FGL96_RS27360 (62283) 62283..63191 + 909 WP_023302803.1 HNH endonuclease -
FGL96_RS27365 (63578) 63578..63928 - 351 WP_004213569.1 DUF305 domain-containing protein -
FGL96_RS27370 (64072) 64072..64503 - 432 WP_023302802.1 silver-binding protein SilE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA 1..270854 270854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T126888 WP_001159871.1 NZ_CP040712:c59856-59551 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T126888 NZ_CP040712:c59856-59551 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT126888 WP_000813634.1 NZ_CP040712:c60076-59858 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT126888 NZ_CP040712:c60076-59858 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References