Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 59551..60076 Replicon plasmid p16HN-35_Vir
Accession NZ_CP040712
Organism Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag FGL96_RS27345 Protein ID WP_001159871.1
Coordinates 59551..59856 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag FGL96_RS27350 Protein ID WP_000813634.1
Coordinates 59858..60076 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL96_RS27310 (54978) 54978..55775 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
FGL96_RS27315 (55837) 55837..56088 + 252 Protein_74 DUF4113 domain-containing protein -
FGL96_RS27320 (56093) 56093..56485 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
FGL96_RS27325 (56490) 56490..57461 - 972 WP_001103694.1 plasmid segregation protein ParM -
FGL96_RS27330 (57690) 57690..58334 + 645 WP_000633911.1 AAA family ATPase -
FGL96_RS27335 (58328) 58328..58603 + 276 WP_000239529.1 hypothetical protein -
FGL96_RS27340 (58741) 58741..59550 - 810 WP_000016979.1 site-specific integrase -
FGL96_RS27345 (59551) 59551..59856 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
FGL96_RS27350 (59858) 59858..60076 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
FGL96_RS27355 (60672) 60672..61646 - 975 WP_004213565.1 sensor domain-containing diguanylate cyclase -
FGL96_RS27360 (62283) 62283..63191 + 909 WP_023302803.1 HNH endonuclease -
FGL96_RS27365 (63578) 63578..63928 - 351 WP_004213569.1 DUF305 domain-containing protein -
FGL96_RS27370 (64072) 64072..64503 - 432 WP_023302802.1 silver-binding protein SilE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / qacE / sul1 / mph(A) iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA 1..270854 270854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T126888 WP_001159871.1 NZ_CP040712:c59856-59551 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T126888 NZ_CP040712:c59856-59551 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT126888 WP_000813634.1 NZ_CP040712:c60076-59858 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT126888 NZ_CP040712:c60076-59858 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References