Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1703041..1703660 Replicon chromosome
Accession NZ_CP040711
Organism Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag FGL96_RS08095 Protein ID WP_002892050.1
Coordinates 1703041..1703259 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag FGL96_RS08100 Protein ID WP_002892066.1
Coordinates 1703286..1703660 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL96_RS08060 (FGL96_08140) 1699088..1699351 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
FGL96_RS08065 (FGL96_08145) 1699351..1699491 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
FGL96_RS08070 (FGL96_08150) 1699488..1700186 - 699 WP_002892021.1 GNAT family protein -
FGL96_RS08075 (FGL96_08155) 1700287..1701738 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
FGL96_RS08080 (FGL96_08160) 1701713..1702183 - 471 WP_002892026.1 YlaC family protein -
FGL96_RS08085 1702204..1702344 + 141 WP_004147370.1 hypothetical protein -
FGL96_RS08090 (FGL96_08165) 1702316..1702882 - 567 WP_002892030.1 maltose O-acetyltransferase -
FGL96_RS08095 (FGL96_08170) 1703041..1703259 - 219 WP_002892050.1 HHA domain-containing protein Toxin
FGL96_RS08100 (FGL96_08175) 1703286..1703660 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
FGL96_RS08105 (FGL96_08180) 1704146..1707292 - 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
FGL96_RS08110 (FGL96_08185) 1707315..1708508 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T126873 WP_002892050.1 NZ_CP040711:c1703259-1703041 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T126873 NZ_CP040711:c1703259-1703041 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT126873 WP_002892066.1 NZ_CP040711:c1703660-1703286 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT126873 NZ_CP040711:c1703660-1703286 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References