Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 178518..179143 Replicon chromosome
Accession NZ_CP040711
Organism Klebsiella pneumoniae strain 16HN-35

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag FGL96_RS00870 Protein ID WP_002882817.1
Coordinates 178518..178901 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag FGL96_RS00875 Protein ID WP_004150355.1
Coordinates 178901..179143 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGL96_RS00855 (FGL96_00870) 175884..176786 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
FGL96_RS00860 (FGL96_00875) 176783..177418 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
FGL96_RS00865 (FGL96_00880) 177415..178344 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
FGL96_RS00870 (FGL96_00885) 178518..178901 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
FGL96_RS00875 (FGL96_00890) 178901..179143 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
FGL96_RS00880 (FGL96_00895) 179348..180265 + 918 WP_002882812.1 alpha/beta hydrolase -
FGL96_RS00885 (FGL96_00900) 180279..181220 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
FGL96_RS00890 (FGL96_00905) 181265..181702 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
FGL96_RS00895 (FGL96_00910) 181699..182559 - 861 WP_002882807.1 virulence factor BrkB family protein -
FGL96_RS00900 (FGL96_00915) 182553..183152 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T126867 WP_002882817.1 NZ_CP040711:c178901-178518 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T126867 NZ_CP040711:c178901-178518 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT126867 WP_004150355.1 NZ_CP040711:c179143-178901 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT126867 NZ_CP040711:c179143-178901 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References