Detailed information of TA system TA126864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 18835..19354
• Replicon: plasmid p2
• Accession: NZ_CP040708
• Organism: Enterococcus faecium strain HOU503

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: FGF98_RS15240
• Protein ID: WP_000588503.1
• Coordinates: 19097..19354 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: FGF98_RS15235
• Protein ID: WP_000388479.1
• Coordinates: 18835..19104 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FGF98_RS15190	14229..14909	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
FGF98_RS15195	15096..15512	-	417	WP_001232859.1	hypothetical protein	-
FGF98_RS15200	15515..15799	-	285	WP_000353844.1	hypothetical protein	-
FGF98_RS15205	16145..16390	+	246	WP_002311817.1	hypothetical protein	-
FGF98_RS15210	16692..16763	-	72	WP_138816312.1	type I toxin-antitoxin system Fst family toxin	-
FGF98_RS15215	16822..17502	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
FGF98_RS15220	17578..18144	+	567	Protein_20	hypothetical protein	-
FGF98_RS15230	18525..18704	+	180	WP_001120991.1	hypothetical protein	-
FGF98_RS15235	18835..19104	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FGF98_RS15240	19097..19354	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
FGF98_RS15245	19992..20816	+	825	WP_001809248.1	hypothetical protein	-
FGF98_RS15250	20981..21595	-	615	WP_001261742.1	recombinase family protein	-
FGF98_RS15255	22045..23370	+	1326	WP_000969590.1	Y-family DNA polymerase	-
FGF98_RS15260	23363..23713	+	351	WP_000568378.1	hypothetical protein	-
FGF98_RS15265	23710..23889	+	180	WP_001021554.1	hypothetical protein	-
FGF98_RS15270	24025..24315	+	291	WP_000026576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / erm(B) / ant(6)-Ia / aph(3')-III	-	1..71214	71214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T126864 WP_000588503.1 NZ_CP040708:19097-19354 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T126864 NZ_CP040708:19097-19354 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT126864 WP_000388479.1 NZ_CP040708:18835-19104 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT126864 NZ_CP040708:18835-19104 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7