Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2175951..2176522 Replicon chromosome
Accession NZ_CP040706
Organism Enterococcus faecium strain HOU503

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag FGF98_RS12265 Protein ID WP_002286801.1
Coordinates 2176181..2176522 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag FGF98_RS12260 Protein ID WP_002323011.1
Coordinates 2175951..2176181 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FGF98_RS12210 2171087..2172415 + 1329 WP_008265691.1 FAD-containing oxidoreductase -
FGF98_RS12215 2172437..2173063 + 627 WP_002286816.1 cysteine hydrolase -
FGF98_RS12220 2173246..2173827 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
FGF98_RS12240 2174559..2174906 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
FGF98_RS12245 2174882..2175121 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
FGF98_RS12250 2175326..2175664 - 339 WP_002286804.1 hypothetical protein -
FGF98_RS12260 2175951..2176181 + 231 WP_002323011.1 hypothetical protein Antitoxin
FGF98_RS12265 2176181..2176522 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FGF98_RS12270 2177555..2178742 - 1188 WP_002296840.1 IS256-like element IS16 family transposase -
FGF98_RS12275 2179536..2179730 + 195 WP_002297028.1 hypothetical protein -
FGF98_RS12280 2179888..2180170 + 283 Protein_1997 transposase -
FGF98_RS12290 2180414..2181244 - 831 WP_002286789.1 manganese catalase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2177555..2178742 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T126863 WP_002286801.1 NZ_CP040706:2176181-2176522 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T126863 NZ_CP040706:2176181-2176522 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT126863 WP_002323011.1 NZ_CP040706:2175951-2176181 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT126863 NZ_CP040706:2175951-2176181 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References