Detailed information of TA system TA126853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3171478..3172165
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: FF953_RS15125
• Protein ID: WP_003414624.1
• Coordinates: 3171722..3172165 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: FF953_RS15120
• Protein ID: WP_003414620.1
• Coordinates: 3171478..3171735 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS15100 (FF953_15630)	3166798..3167652	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FF953_RS15105 (FF953_15635)	3167708..3168871	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FF953_RS15110 (FF953_15640)	3168888..3170102	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FF953_RS15115 (FF953_15645)	3170110..3171351	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
FF953_RS15120 (FF953_15650)	3171478..3171735	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
FF953_RS15125 (FF953_15655)	3171722..3172165	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF953_RS15130 (FF953_15660)	3172245..3172907	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
FF953_RS15135 (FF953_15665)	3173003..3173194	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FF953_RS15140 (FF953_15670)	3173526..3175274	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
FF953_RS15145 (FF953_15675)	3175370..3175951	+	582	WP_003414644.1	fasciclin domain-containing protein	-
FF953_RS15150 (FF953_15680)	3176051..3176317	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T126853 WP_003414624.1 NZ_CP040693:3171722-3172165 [Mycobacterium tuberculosis complex sp. N0145]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T126853 NZ_CP040693:3171722-3172165 [Mycobacterium tuberculosis complex sp. N0145]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT126853 WP_003414620.1 NZ_CP040693:3171478-3171735 [Mycobacterium tuberculosis complex sp. N0145]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT126853 NZ_CP040693:3171478-3171735 [Mycobacterium tuberculosis complex sp. N0145]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0