Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PIN(toxin) |
| Location | 2865431..2866068 | Replicon | chromosome |
| Accession | NZ_CP040693 | ||
| Organism | Mycobacterium tuberculosis complex sp. N0145 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P0DMV7 |
| Locus tag | FF953_RS13455 | Protein ID | WP_003413180.1 |
| Coordinates | 2865431..2865826 (-) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ44 |
| Locus tag | FF953_RS13460 | Protein ID | WP_003413183.1 |
| Coordinates | 2865823..2866068 (-) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FF953_RS13415 (FF953_13890) | 2860835..2862046 | + | 1212 | WP_003900845.1 | alpha/beta hydrolase family protein | - |
| FF953_RS13420 (FF953_13900) | 2862173..2862832 | + | 660 | WP_003902296.1 | LppA family lipoprotein | - |
| FF953_RS13425 (FF953_13905) | 2862829..2863491 | + | 663 | WP_003905878.1 | LppA family lipoprotein | - |
| FF953_RS13430 (FF953_13910) | 2863488..2863765 | + | 278 | Protein_2651 | type II toxin-antitoxin system VapB family antitoxin | - |
| FF953_RS13435 (FF953_13915) | 2863858..2864271 | + | 414 | WP_003413164.1 | PIN domain nuclease | - |
| FF953_RS13440 (FF953_13920) | 2864310..2864567 | + | 258 | WP_003413167.1 | CopG family transcriptional regulator | - |
| FF953_RS13445 (FF953_13925) | 2864564..2864941 | + | 378 | WP_003413174.1 | type II toxin-antitoxin system VapC family toxin | - |
| FF953_RS13450 (FF953_13930) | 2864957..2865331 | - | 375 | WP_003413177.1 | hypothetical protein | - |
| FF953_RS13455 (FF953_13935) | 2865431..2865826 | - | 396 | WP_003413180.1 | type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 | Toxin |
| FF953_RS13460 (FF953_13940) | 2865823..2866068 | - | 246 | WP_003413183.1 | type II toxin-antitoxin system antitoxin VapB20 | Antitoxin |
| FF953_RS13465 (FF953_13950) | 2866479..2866898 | - | 420 | WP_003413190.1 | A24 family peptidase | - |
| FF953_RS13470 (FF953_13955) | 2866910..2867719 | - | 810 | WP_003413193.1 | shikimate dehydrogenase | - |
| FF953_RS13475 (FF953_13960) | 2867716..2868969 | - | 1254 | WP_003413196.1 | endolytic transglycosylase MltG | - |
| FF953_RS13480 (FF953_13965) | 2868962..2869474 | - | 513 | WP_003413197.1 | Holliday junction resolvase RuvX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 14619.53 Da Isoelectric Point: 6.9794
>T126844 WP_003413180.1 NZ_CP040693:c2865826-2865431 [Mycobacterium tuberculosis complex sp. N0145]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
Download Length: 396 bp
>T126844 NZ_CP040693:c2865826-2865431 [Mycobacterium tuberculosis complex sp. N0145]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
Antitoxin
Download Length: 82 a.a. Molecular weight: 9098.33 Da Isoelectric Point: 5.6007
>AT126844 WP_003413183.1 NZ_CP040693:c2866068-2865823 [Mycobacterium tuberculosis complex sp. N0145]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
Download Length: 246 bp
>AT126844 NZ_CP040693:c2866068-2865823 [Mycobacterium tuberculosis complex sp. N0145]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 5WZ4 | |
| PDB | 5WZF |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BW31 |