Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 2318486..2319122 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name mazF Uniprot ID P0CL62
Locus tag FF953_RS10835 Protein ID WP_003410654.1
Coordinates 2318712..2319122 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag FF953_RS10830 Protein ID WP_003410651.1
Coordinates 2318486..2318719 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS10815 2313934..2314335 + 402 WP_009937711.1 metal ABC transporter permease -
FF953_RS10820 (FF953_11215) 2314336..2314740 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
FF953_RS10825 (FF953_11220) 2314824..2318408 - 3585 WP_003910889.1 cobaltochelatase subunit CobN -
FF953_RS10830 (FF953_11225) 2318486..2318719 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
FF953_RS10835 (FF953_11230) 2318712..2319122 + 411 WP_003410654.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
FF953_RS10840 (FF953_11235) 2319106..2320197 + 1092 WP_003900454.1 precorrin-3B synthase -
FF953_RS10845 (FF953_11240) 2320207..2320833 + 627 WP_003410658.1 precorrin-8X methylmutase -
FF953_RS10850 (FF953_11245) 2320830..2322356 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
FF953_RS10855 (FF953_11250) 2322302..2323525 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14235.42 Da        Isoelectric Point: 10.6228

>T126837 WP_003410654.1 NZ_CP040693:2318712-2319122 [Mycobacterium tuberculosis complex sp. N0145]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T126837 NZ_CP040693:2318712-2319122 [Mycobacterium tuberculosis complex sp. N0145]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT126837 WP_003410651.1 NZ_CP040693:2318486-2318719 [Mycobacterium tuberculosis complex sp. N0145]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT126837 NZ_CP040693:2318486-2318719 [Mycobacterium tuberculosis complex sp. N0145]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7DU4
PDB 5WYG
PDB 6A6X

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References