Detailed information of TA system TA126835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2249343..2249969
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FF953_RS10535
• Protein ID: WP_003410075.1
• Coordinates: 2249571..2249969 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FF953_RS10530
• Protein ID: WP_003911750.1
• Coordinates: 2249343..2249570 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS10520 (FF953_10900)	2247382..2247726	-	345	WP_003410065.1	ferredoxin family protein	-
FF953_RS10525 (FF953_10905)	2247915..2249084	-	1170	WP_003899126.1	ATP-binding protein	-
FF953_RS10530 (FF953_10910)	2249343..2249570	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF953_RS10535 (FF953_10915)	2249571..2249969	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FF953_RS10540 (FF953_10920)	2250152..2250583	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FF953_RS10545 (FF953_10925)	2250684..2251118	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
FF953_RS10550 (FF953_10940)	2251555..2251734	-	180	Protein_2085	hypothetical protein	-
FF953_RS10555 (FF953_10945)	2251822..2252442	+	621	WP_003410086.1	IS110 family transposase	-
FF953_RS10560 (FF953_10950)	2252396..2252986	+	591	WP_003899131.1	IS110 family transposase	-
FF953_RS10565 (FF953_10955)	2253114..2254370	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
FF953_RS10570 (FF953_10965)	2254724..2254924	+	201	Protein_2089	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T126835 WP_003410075.1 NZ_CP040693:2249571-2249969 [Mycobacterium tuberculosis complex sp. N0145]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T126835 NZ_CP040693:2249571-2249969 [Mycobacterium tuberculosis complex sp. N0145]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT126835 WP_003911750.1 NZ_CP040693:2249343-2249570 [Mycobacterium tuberculosis complex sp. N0145]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT126835 NZ_CP040693:2249343-2249570 [Mycobacterium tuberculosis complex sp. N0145]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8