Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2216712..2217400 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag FF953_RS10365 Protein ID WP_003409958.1
Coordinates 2216712..2217131 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag FF953_RS10370 Protein ID WP_003409968.1
Coordinates 2217140..2217400 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS10345 (FF953_10725) 2212207..2213055 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
FF953_RS10350 (FF953_10730) 2213018..2214463 - 1446 WP_003899115.1 APC family permease -
FF953_RS10355 (FF953_10735) 2214642..2215328 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
FF953_RS10360 (FF953_10740) 2215519..2216487 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
FF953_RS10365 (FF953_10745) 2216712..2217131 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
FF953_RS10370 (FF953_10750) 2217140..2217400 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FF953_RS10375 (FF953_10755) 2217543..2219219 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
FF953_RS10380 (FF953_10760) 2219207..2219860 - 654 WP_003409976.1 cutinase Cfp21 -
FF953_RS10385 (FF953_10765) 2219975..2220205 + 231 WP_003409980.1 DUF167 domain-containing protein -
FF953_RS10390 (FF953_10770) 2220290..2221201 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
FF953_RS10395 (FF953_10775) 2221310..2221909 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T126832 WP_003409958.1 NZ_CP040693:c2217131-2216712 [Mycobacterium tuberculosis complex sp. N0145]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T126832 NZ_CP040693:c2217131-2216712 [Mycobacterium tuberculosis complex sp. N0145]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT126832 WP_003409968.1 NZ_CP040693:c2217400-2217140 [Mycobacterium tuberculosis complex sp. N0145]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT126832 NZ_CP040693:c2217400-2217140 [Mycobacterium tuberculosis complex sp. N0145]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References