Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1941271..1941884 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag FF953_RS09040 Protein ID WP_003408465.1
Coordinates 1941271..1941660 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag FF953_RS09045 Protein ID WP_003408469.1
Coordinates 1941657..1941884 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS09005 (FF953_09310) 1936900..1937814 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
FF953_RS09010 (FF953_09315) 1937817..1938647 + 831 WP_003408448.1 cyclase family protein -
FF953_RS09015 (FF953_09320) 1938647..1938997 + 351 WP_003898986.1 cupin domain-containing protein -
FF953_RS09020 (FF953_09325) 1939050..1939868 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
FF953_RS09025 (FF953_09330) 1939882..1940661 + 780 WP_003408460.1 IclR family transcriptional regulator -
FF953_RS09035 1941092..1941223 + 132 Protein_1782 IS3 family transposase -
FF953_RS09040 (FF953_09340) 1941271..1941660 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
FF953_RS09045 (FF953_09345) 1941657..1941884 - 228 WP_003408469.1 antitoxin Antitoxin
FF953_RS09050 (FF953_09350) 1942102..1943586 + 1485 WP_003408473.1 biotin carboxylase -
FF953_RS09055 (FF953_09355) 1943583..1944830 + 1248 WP_003408476.1 serine hydrolase -
FF953_RS09060 (FF953_09360) 1944873..1945292 - 420 WP_003408483.1 hypothetical protein -
FF953_RS09065 (FF953_09365) 1945282..1945992 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T126827 WP_003408465.1 NZ_CP040693:c1941660-1941271 [Mycobacterium tuberculosis complex sp. N0145]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T126827 NZ_CP040693:c1941660-1941271 [Mycobacterium tuberculosis complex sp. N0145]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGCCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT126827 WP_003408469.1 NZ_CP040693:c1941884-1941657 [Mycobacterium tuberculosis complex sp. N0145]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT126827 NZ_CP040693:c1941884-1941657 [Mycobacterium tuberculosis complex sp. N0145]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References