Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1690857..1691473 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag FF953_RS07945 Protein ID WP_003407593.1
Coordinates 1691156..1691473 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag FF953_RS07940 Protein ID WP_003900349.1
Coordinates 1690857..1691159 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS07930 (FF953_08210) 1686743..1688590 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
FF953_RS07935 (FF953_08215) 1688591..1690843 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
FF953_RS07940 (FF953_08220) 1690857..1691159 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
FF953_RS07945 (FF953_08225) 1691156..1691473 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
FF953_RS07950 (FF953_08230) 1691470..1692474 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
FF953_RS07955 (FF953_08235) 1692527..1693816 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
FF953_RS07960 (FF953_08240) 1693889..1694614 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
FF953_RS07965 (FF953_08245) 1694720..1694932 - 213 WP_003898900.1 dodecin family protein -
FF953_RS07970 (FF953_08250) 1694993..1695391 + 399 WP_003900351.1 hypothetical protein -
FF953_RS07975 (FF953_08255) 1695436..1696464 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T126825 WP_003407593.1 NZ_CP040693:1691156-1691473 [Mycobacterium tuberculosis complex sp. N0145]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T126825 NZ_CP040693:1691156-1691473 [Mycobacterium tuberculosis complex sp. N0145]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT126825 WP_003900349.1 NZ_CP040693:1690857-1691159 [Mycobacterium tuberculosis complex sp. N0145]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT126825 NZ_CP040693:1690857-1691159 [Mycobacterium tuberculosis complex sp. N0145]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References