Detailed information of TA system TA126821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1385577..1386265
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: FF953_RS06605
• Protein ID: WP_003406304.1
• Coordinates: 1385834..1386265 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: FF953_RS06600
• Protein ID: WP_003406302.1
• Coordinates: 1385577..1385837 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS06580 (FF953_06785)	1381154..1381978	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
FF953_RS06585 (FF953_06790)	1381983..1383164	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
FF953_RS06590 (FF953_06795)	1383241..1384341	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
FF953_RS06595 (FF953_06800)	1384512..1385501	+	990	WP_003406301.1	malate dehydrogenase	-
FF953_RS06600 (FF953_06805)	1385577..1385837	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
FF953_RS06605 (FF953_06810)	1385834..1386265	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF953_RS06610 (FF953_06815)	1386288..1387871	-	1584	WP_009940130.1	PE family protein	-
FF953_RS06615 (FF953_06825)	1388051..1388911	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
FF953_RS06620 (FF953_06830)	1388992..1389822	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
FF953_RS06625 (FF953_06835)	1389879..1390172	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
FF953_RS06630 (FF953_06840)	1390169..1390438	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T126821 WP_003406304.1 NZ_CP040693:1385834-1386265 [Mycobacterium tuberculosis complex sp. N0145]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T126821 NZ_CP040693:1385834-1386265 [Mycobacterium tuberculosis complex sp. N0145]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT126821 WP_003406302.1 NZ_CP040693:1385577-1385837 [Mycobacterium tuberculosis complex sp. N0145]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT126821 NZ_CP040693:1385577-1385837 [Mycobacterium tuberculosis complex sp. N0145]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6