Detailed information of TA system TA126820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239354..1239922
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FF953_RS05930
• Protein ID: WP_003405865.1
• Coordinates: 1239548..1239922 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FF953_RS05925
• Protein ID: WP_003405863.1
• Coordinates: 1239354..1239551 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS05905 (FF953_06085)	1235395..1236033	-	639	WP_003898730.1	lipid droplet-associated protein	-
FF953_RS05910 (FF953_06090)	1236123..1237130	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FF953_RS05915 (FF953_06095)	1237147..1238130	-	984	WP_003898731.1	hypothetical protein	-
FF953_RS05920 (FF953_06100)	1238193..1239266	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FF953_RS05925 (FF953_06105)	1239354..1239551	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF953_RS05930 (FF953_06110)	1239548..1239922	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF953_RS05935 (FF953_06115)	1240125..1240823	+	699	WP_003898733.1	hypothetical protein	-
FF953_RS05940 (FF953_06120)	1240941..1241126	+	186	WP_003901093.1	hypothetical protein	-
FF953_RS05945 (FF953_06125)	1241053..1241328	-	276	WP_003405867.1	hypothetical protein	-
FF953_RS05950 (FF953_06130)	1241571..1241894	+	324	WP_003405871.1	putative quinol monooxygenase	-
FF953_RS05955 (FF953_06135)	1241909..1242607	-	699	WP_031646953.1	hypothetical protein	-
FF953_RS05960	1242641..1242850	+	210	WP_003911400.1	hypothetical protein	-
FF953_RS05965 (FF953_06140)	1242802..1243572	-	771	Protein_1175	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T126820 WP_003405865.1 NZ_CP040693:1239548-1239922 [Mycobacterium tuberculosis complex sp. N0145]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T126820 NZ_CP040693:1239548-1239922 [Mycobacterium tuberculosis complex sp. N0145]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT126820 WP_003405863.1 NZ_CP040693:1239354-1239551 [Mycobacterium tuberculosis complex sp. N0145]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT126820 NZ_CP040693:1239354-1239551 [Mycobacterium tuberculosis complex sp. N0145]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07