Detailed information of TA system TA126817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1013065..1013684
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: FF953_RS04790
• Protein ID: WP_003404726.1
• Coordinates: 1013250..1013684 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: FF953_RS04785
• Protein ID: WP_003898641.1
• Coordinates: 1013065..1013244 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS04770 (FF953_04930)	1008538..1010118	+	1581	WP_003910977.1	serine hydrolase	-
FF953_RS04775 (FF953_04935)	1010115..1012508	+	2394	WP_031715176.1	cation-translocating P-type ATPase	-
FF953_RS04780	1012781..1012939	+	159	WP_003404720.1	hypothetical protein	-
FF953_RS04785 (FF953_04940)	1013065..1013244	+	180	WP_003898641.1	antitoxin	Antitoxin
FF953_RS04790 (FF953_04945)	1013250..1013684	+	435	WP_003404726.1	SRPBCC family protein	Toxin
FF953_RS04795 (FF953_04950)	1013782..1014555	+	774	WP_003404735.1	VOC family protein	-
FF953_RS04800 (FF953_04955)	1014620..1015069	+	450	WP_003404738.1	hypothetical protein	-
FF953_RS04805 (FF953_04960)	1015204..1015434	-	231	WP_003898642.1	hypothetical protein	-
FF953_RS04810 (FF953_04965)	1015601..1017109	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
FF953_RS04815 (FF953_04970)	1017111..1018349	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T126817 WP_003404726.1 NZ_CP040693:1013250-1013684 [Mycobacterium tuberculosis complex sp. N0145]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T126817 NZ_CP040693:1013250-1013684 [Mycobacterium tuberculosis complex sp. N0145]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT126817 WP_003898641.1 NZ_CP040693:1013065-1013244 [Mycobacterium tuberculosis complex sp. N0145]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT126817 NZ_CP040693:1013065-1013244 [Mycobacterium tuberculosis complex sp. N0145]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5