Detailed information of TA system TA126812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 750812..751445
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: FF953_RS03440
• Protein ID: WP_003403365.1
• Coordinates: 750812..751195 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FF953_RS03445
• Protein ID: WP_003403368.1
• Coordinates: 751290..751445 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS03415 (FF953_03495)	746104..746640	+	537	WP_003403341.1	50S ribosomal protein L10	-
FF953_RS03420 (FF953_03500)	746677..747069	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FF953_RS03425 (FF953_03505)	747062..747757	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FF953_RS03430 (FF953_03510)	747828..749333	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF953_RS03435 (FF953_03515)	749414..750424	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
FF953_RS03440 (FF953_03520)	750812..751195	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
FF953_RS03445 (FF953_03525)	751290..751445	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF953_RS03450 (FF953_03530)	751521..752237	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
FF953_RS03455 (FF953_03535)	752513..752821	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FF953_RS03460 (FF953_03540)	752808..753053	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FF953_RS03465 (FF953_03545)	753163..753600	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF953_RS03470 (FF953_03550)	753597..753851	-	255	WP_003911263.1	antitoxin VapB7	-
FF953_RS03475 (FF953_03555)	753965..756328	+	2364	WP_003901895.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T126812 WP_003403365.1 NZ_CP040693:c751195-750812 [Mycobacterium tuberculosis complex sp. N0145]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T126812 NZ_CP040693:c751195-750812 [Mycobacterium tuberculosis complex sp. N0145]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT126812 WP_003403368.1 NZ_CP040693:c751445-751290 [Mycobacterium tuberculosis complex sp. N0145]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT126812 NZ_CP040693:c751445-751290 [Mycobacterium tuberculosis complex sp. N0145]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8