Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 714991..715640 Replicon chromosome
Accession NZ_CP040693
Organism Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag FF953_RS03260 Protein ID WP_003403236.1
Coordinates 715245..715640 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag FF953_RS03255 Protein ID WP_003403235.1
Coordinates 714991..715245 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF953_RS03230 710117..710200 + 84 Protein_640 galactose-1-phosphate uridylyltransferase -
FF953_RS03235 710219..711300 + 1082 Protein_641 galactose-1-phosphate uridylyltransferase -
FF953_RS03240 (FF953_03315) 711297..712388 + 1092 WP_003900977.1 galactokinase -
FF953_RS03245 (FF953_03325) 712783..713847 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
FF953_RS03250 (FF953_03330) 713951..714898 + 948 WP_003403232.1 DUF732 domain-containing protein -
FF953_RS03255 (FF953_03335) 714991..715245 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
FF953_RS03260 (FF953_03340) 715245..715640 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
FF953_RS03265 (FF953_03345) 715734..716474 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
FF953_RS03270 (FF953_03350) 716606..716866 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
FF953_RS03275 (FF953_03355) 716863..717270 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
FF953_RS03280 (FF953_03360) 717342..718493 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
FF953_RS03285 (FF953_03365) 718586..720313 - 1728 WP_003901883.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T126810 WP_003403236.1 NZ_CP040693:715245-715640 [Mycobacterium tuberculosis complex sp. N0145]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T126810 NZ_CP040693:715245-715640 [Mycobacterium tuberculosis complex sp. N0145]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT126810 WP_003403235.1 NZ_CP040693:714991-715245 [Mycobacterium tuberculosis complex sp. N0145]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT126810 NZ_CP040693:714991-715245 [Mycobacterium tuberculosis complex sp. N0145]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References