Detailed information of TA system TA126804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 638814..639490
• Replicon: chromosome
• Accession: NZ_CP040693
• Organism: Mycobacterium tuberculosis complex sp. N0145

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: FF953_RS02870
• Protein ID: WP_003898500.1
• Coordinates: 638814..639227 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: FF953_RS02875
• Protein ID: WP_003402915.1
• Coordinates: 639224..639490 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF953_RS02840 (FF953_02885)	634159..634461	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
FF953_RS02845 (FF953_02890)	634521..634799	-	279	WP_003402901.1	hypothetical protein	-
FF953_RS02850 (FF953_02895)	634796..636049	-	1254	WP_003402904.1	inorganic phosphate transporter	-
FF953_RS02855 (FF953_02900)	636169..636555	-	387	WP_003402907.1	VOC family protein	-
FF953_RS02860 (FF953_02905)	636618..637502	-	885	WP_003402909.1	SDR family oxidoreductase	-
FF953_RS02865 (FF953_02910)	637598..638500	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
FF953_RS02870 (FF953_02915)	638814..639227	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF953_RS02875 (FF953_02920)	639224..639490	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
FF953_RS02880 (FF953_02925)	639682..641397	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
FF953_RS02885 (FF953_02930)	641475..643079	+	1605	WP_003402920.1	amidohydrolase	-
FF953_RS02890 (FF953_02935)	643076..644056	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T126804 WP_003898500.1 NZ_CP040693:c639227-638814 [Mycobacterium tuberculosis complex sp. N0145]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T126804 NZ_CP040693:c639227-638814 [Mycobacterium tuberculosis complex sp. N0145]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT126804 WP_003402915.1 NZ_CP040693:c639490-639224 [Mycobacterium tuberculosis complex sp. N0145]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT126804 NZ_CP040693:c639490-639224 [Mycobacterium tuberculosis complex sp. N0145]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0