Detailed information of TA system TA126796

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 4043648..4044219
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FF956_RS19000
• Protein ID: WP_003401560.1
• Coordinates: 4043917..4044219 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FF956_RS18995
• Protein ID: WP_003401563.1
• Coordinates: 4043648..4043869 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS18980 (FF956_19825)	4041553..4042461	-	909	WP_003900117.1	protochlorophyllide reductase	-
FF956_RS18985 (FF956_19830)	4042458..4043090	-	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
FF956_RS18990 (FF956_19835)	4043226..4043651	-	426	WP_003401566.1	PIN domain nuclease	-
FF956_RS18995 (FF956_19840)	4043648..4043869	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FF956_RS19000 (FF956_19845)	4043917..4044219	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FF956_RS19005 (FF956_19850)	4044216..4044443	-	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FF956_RS19010 (FF956_19855)	4044586..4046232	-	1647	WP_031654185.1	PE family protein	-
FF956_RS19015 (FF956_19860)	4046411..4047808	+	1398	Protein_3754	sulfatase	-
FF956_RS19020 (FF956_19865)	4047818..4048621	+	804	WP_003401540.1	Stf0 family sulphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T126796 WP_003401560.1 NZ_CP040692:c4044219-4043917 [Mycobacterium tuberculosis complex sp. N0157]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T126796 NZ_CP040692:c4044219-4043917 [Mycobacterium tuberculosis complex sp. N0157]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT126796 WP_003401563.1 NZ_CP040692:c4043869-4043648 [Mycobacterium tuberculosis complex sp. N0157]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT126796 NZ_CP040692:c4043869-4043648 [Mycobacterium tuberculosis complex sp. N0157]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48