Detailed information of TA system TA126785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3662631..3663264
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: FF956_RS17165
• Protein ID: WP_003403365.1
• Coordinates: 3662881..3663264 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: FF956_RS17160
• Protein ID: WP_003403368.1
• Coordinates: 3662631..3662786 (+)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS17130 (FF956_17910)	3657748..3660111	-	2364	WP_003903123.1	arylsulfatase AtsD	-
FF956_RS17135 (FF956_17915)	3660225..3660479	+	255	WP_003911263.1	antitoxin VapB7	-
FF956_RS17140 (FF956_17920)	3660476..3660913	+	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF956_RS17145 (FF956_17925)	3661023..3661268	+	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
FF956_RS17150 (FF956_17930)	3661255..3661563	+	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FF956_RS17155 (FF956_17935)	3661839..3662555	+	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF956_RS17160 (FF956_17940)	3662631..3662786	+	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF956_RS17165 (FF956_17945)	3662881..3663264	+	384	WP_003403365.1	ribonuclease VapC6	Toxin
FF956_RS17170 (FF956_17950)	3663652..3664662	-	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF956_RS17175 (FF956_17955)	3664743..3666248	-	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
FF956_RS17180 (FF956_17960)	3666319..3667014	+	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
FF956_RS17185 (FF956_17965)	3667007..3667399	-	393	WP_003403353.1	50S ribosomal protein L7/L12	-
FF956_RS17190 (FF956_17970)	3667436..3667972	-	537	WP_003403341.1	50S ribosomal protein L10	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T126785 WP_003403365.1 NZ_CP040692:3662881-3663264 [Mycobacterium tuberculosis complex sp. N0157]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T126785 NZ_CP040692:3662881-3663264 [Mycobacterium tuberculosis complex sp. N0157]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT126785 WP_003403368.1 NZ_CP040692:3662631-3662786 [Mycobacterium tuberculosis complex sp. N0157]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT126785 NZ_CP040692:3662631-3662786 [Mycobacterium tuberculosis complex sp. N0157]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8