Detailed information of TA system TA126784

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3661023..3661563
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P9WII0
• Locus tag: FF956_RS17150
• Protein ID: WP_003403376.1
• Coordinates: 3661255..3661563 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: FF956_RS17145
• Protein ID: WP_003403381.1
• Coordinates: 3661023..3661268 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS17115 (FF956_17895)	3656939..3657112	-	174	WP_003898549.1	hypothetical protein	-
FF956_RS17120 (FF956_17900)	3657109..3657447	-	339	WP_003403405.1	PIN domain-containing protein	-
FF956_RS17125 (FF956_17905)	3657359..3657685	-	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FF956_RS17130 (FF956_17910)	3657748..3660111	-	2364	WP_003903123.1	arylsulfatase AtsD	-
FF956_RS17135 (FF956_17915)	3660225..3660479	+	255	WP_003911263.1	antitoxin VapB7	-
FF956_RS17140 (FF956_17920)	3660476..3660913	+	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
FF956_RS17145 (FF956_17925)	3661023..3661268	+	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
FF956_RS17150 (FF956_17930)	3661255..3661563	+	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FF956_RS17155 (FF956_17935)	3661839..3662555	+	717	WP_003903122.1	CPBP family intramembrane metalloprotease	-
FF956_RS17160 (FF956_17940)	3662631..3662786	+	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
FF956_RS17165 (FF956_17945)	3662881..3663264	+	384	WP_003403365.1	ribonuclease VapC6	-
FF956_RS17170 (FF956_17950)	3663652..3664662	-	1011	WP_031653722.1	ABC transporter ATP-binding protein	-
FF956_RS17175 (FF956_17955)	3664743..3666248	-	1506	WP_003403360.1	carotenoid cleavage oxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-101)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11320.10 Da
• Isoelectric Point: 11.6554

>T126784 WP_003403376.1 NZ_CP040692:3661255-3661563 [Mycobacterium tuberculosis complex sp. N0157]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Nucleotide

• Length: 309 bp

>T126784 NZ_CP040692:3661255-3661563 [Mycobacterium tuberculosis complex sp. N0157]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT126784 WP_003403381.1 NZ_CP040692:3661023-3661268 [Mycobacterium tuberculosis complex sp. N0157]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT126784 NZ_CP040692:3661023-3661268 [Mycobacterium tuberculosis complex sp. N0157]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSE5

Antitoxin

• AlphaFold DB: G0TQE0