Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3185318..3185949 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag FF956_RS14770 Protein ID WP_003405820.1
Coordinates 3185638..3185949 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag FF956_RS14765 Protein ID WP_003405836.1
Coordinates 3185318..3185638 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS14740 (FF956_15390) 3180514..3181152 + 639 WP_003898730.1 lipid droplet-associated protein -
FF956_RS14745 (FF956_15395) 3181149..3182396 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
FF956_RS14750 (FF956_15400) 3182386..3182643 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
FF956_RS14755 (FF956_15405) 3182653..3183765 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
FF956_RS14760 (FF956_15410) 3183772..3185308 - 1537 Protein_2917 carboxylesterase/lipase family protein -
FF956_RS14765 (FF956_15415) 3185318..3185638 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
FF956_RS14770 (FF956_15420) 3185638..3185949 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
FF956_RS14775 (FF956_15425) 3186061..3187218 + 1158 WP_003898727.1 AI-2E family transporter -
FF956_RS14780 (FF956_15430) 3187225..3187869 - 645 WP_003915494.1 DUF4245 domain-containing protein -
FF956_RS14785 (FF956_15435) 3187925..3189013 + 1089 WP_017351234.1 class II fructose-bisphosphatase -
FF956_RS14790 (FF956_15440) 3189044..3190468 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T126777 WP_003405820.1 NZ_CP040692:3185638-3185949 [Mycobacterium tuberculosis complex sp. N0157]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T126777 NZ_CP040692:3185638-3185949 [Mycobacterium tuberculosis complex sp. N0157]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT126777 WP_003405836.1 NZ_CP040692:3185318-3185638 [Mycobacterium tuberculosis complex sp. N0157]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT126777 NZ_CP040692:3185318-3185638 [Mycobacterium tuberculosis complex sp. N0157]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References