Detailed information of TA system TA126776

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3176625..3177193
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: FF956_RS14715
• Protein ID: WP_003405865.1
• Coordinates: 3176625..3176999 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: FF956_RS14720
• Protein ID: WP_003405863.1
• Coordinates: 3176996..3177193 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS14680 (FF956_15335)	3172975..3173745	+	771	Protein_2901	adenylate/guanylate cyclase domain-containing protein	-
FF956_RS14685	3173697..3173906	-	210	WP_003911400.1	hypothetical protein	-
FF956_RS14690 (FF956_15340)	3173940..3174638	+	699	WP_031646953.1	hypothetical protein	-
FF956_RS14695 (FF956_15345)	3174653..3174976	-	324	WP_003405871.1	putative quinol monooxygenase	-
FF956_RS14700 (FF956_15350)	3175219..3175494	+	276	WP_003405867.1	hypothetical protein	-
FF956_RS14705 (FF956_15355)	3175421..3175606	-	186	WP_003901093.1	hypothetical protein	-
FF956_RS14710 (FF956_15360)	3175724..3176422	-	699	WP_003898733.1	hypothetical protein	-
FF956_RS14715 (FF956_15365)	3176625..3176999	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF956_RS14720 (FF956_15370)	3176996..3177193	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF956_RS14725 (FF956_15375)	3177281..3178354	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
FF956_RS14730 (FF956_15380)	3178417..3179400	+	984	WP_003903326.1	hypothetical protein	-
FF956_RS14735 (FF956_15385)	3179417..3180424	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
FF956_RS14740 (FF956_15390)	3180514..3181152	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T126776 WP_003405865.1 NZ_CP040692:c3176999-3176625 [Mycobacterium tuberculosis complex sp. N0157]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T126776 NZ_CP040692:c3176999-3176625 [Mycobacterium tuberculosis complex sp. N0157]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT126776 WP_003405863.1 NZ_CP040692:c3177193-3176996 [Mycobacterium tuberculosis complex sp. N0157]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT126776 NZ_CP040692:c3177193-3176996 [Mycobacterium tuberculosis complex sp. N0157]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07