Detailed information of TA system TA126774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3028028..3028587
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0THS1
• Locus tag: FF956_RS14030
• Protein ID: WP_003898789.1
• Coordinates: 3028294..3028587 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0THS2
• Locus tag: FF956_RS14025
• Protein ID: WP_003406322.1
• Coordinates: 3028028..3028297 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS14015 (FF956_14635)	3023234..3024022	+	789	WP_003903379.1	hypothetical protein	-
FF956_RS14020 (FF956_14640)	3024164..3027859	+	3696	WP_015631286.1	multifunctional oxoglutarate decarboxylase/oxoglutarate dehydrogenase thiamine pyrophosphate-binding subunit/dihydrolipoyllysine-residue succinyltransferase subunit	-
FF956_RS14025 (FF956_14645)	3028028..3028297	+	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FF956_RS14030 (FF956_14650)	3028294..3028587	+	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
FF956_RS14035 (FF956_14655)	3028644..3029474	+	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
FF956_RS14040 (FF956_14660)	3029555..3030415	-	861	WP_003903377.1	glycine betaine ABC transporter substrate-binding protein	-
FF956_RS14045 (FF956_14670)	3030595..3031539	+	945	Protein_2774	PE family protein	-
FF956_RS14050 (FF956_14675)	3031637..3032068	-	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	-
FF956_RS14055 (FF956_14680)	3032065..3032325	-	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	-
FF956_RS14060 (FF956_14685)	3032401..3033390	-	990	WP_003406301.1	malate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (9-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11031.57 Da
• Isoelectric Point: 9.6275

>T126774 WP_003898789.1 NZ_CP040692:3028294-3028587 [Mycobacterium tuberculosis complex sp. N0157]
VSDDHPYHVAITATAARDLQRLPEKIAAACVEFVFGPLLNNPHRLGKPLRNDLEGLHSARRGDYRVVYAIDDGHHRVEII
HIARRSASYRMNPCRPR

Nucleotide

• Length: 294 bp

>T126774 NZ_CP040692:3028294-3028587 [Mycobacterium tuberculosis complex sp. N0157]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9766.97 Da
• Isoelectric Point: 4.8615

>AT126774 WP_003406322.1 NZ_CP040692:3028028-3028297 [Mycobacterium tuberculosis complex sp. N0157]
MAVVPLGEVRNRLSEYVAEVELTHERITITRHGHPAAVLISADDLASIEETLEVLRTPGASEAIREGLADVAAGRFVSND
EIRNRYTAR

Nucleotide

• Length: 270 bp

>AT126774 NZ_CP040692:3028028-3028297 [Mycobacterium tuberculosis complex sp. N0157]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: G0THS1

Antitoxin

• AlphaFold DB: G0THS2