Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2463316..2463929 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag FF956_RS11545 Protein ID WP_003408465.1
Coordinates 2463540..2463929 (+) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag FF956_RS11540 Protein ID WP_003408469.1
Coordinates 2463316..2463543 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS11520 (FF956_12035) 2459208..2459918 + 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -
FF956_RS11525 (FF956_12040) 2459908..2460327 + 420 WP_003408483.1 hypothetical protein -
FF956_RS11530 (FF956_12045) 2460370..2461617 - 1248 WP_003408476.1 serine hydrolase -
FF956_RS11535 (FF956_12050) 2461614..2463098 - 1485 WP_003408473.1 biotin carboxylase -
FF956_RS11540 (FF956_12055) 2463316..2463543 + 228 WP_003408469.1 antitoxin Antitoxin
FF956_RS11545 (FF956_12060) 2463540..2463929 + 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
FF956_RS11550 (FF956_12065) 2464033..2465294 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FF956_RS11560 (FF956_12075) 2465897..2466676 - 780 WP_003408460.1 IclR family transcriptional regulator -
FF956_RS11565 (FF956_12080) 2466690..2467504 - 815 Protein_2284 3-keto-5-aminohexanoate cleavage protein -
FF956_RS11570 (FF956_12085) 2467557..2467907 - 351 WP_003898986.1 cupin domain-containing protein -
FF956_RS11575 (FF956_12090) 2467907..2468737 - 831 WP_003907750.1 cyclase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T126769 WP_003408465.1 NZ_CP040692:2463540-2463929 [Mycobacterium tuberculosis complex sp. N0157]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T126769 NZ_CP040692:2463540-2463929 [Mycobacterium tuberculosis complex sp. N0157]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT126769 WP_003408469.1 NZ_CP040692:2463316-2463543 [Mycobacterium tuberculosis complex sp. N0157]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT126769 NZ_CP040692:2463316-2463543 [Mycobacterium tuberculosis complex sp. N0157]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References