Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2182115..2182803 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag FF956_RS10235 Protein ID WP_003409958.1
Coordinates 2182384..2182803 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag FF956_RS10230 Protein ID WP_003409968.1
Coordinates 2182115..2182375 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS10205 (FF956_10635) 2177633..2178232 - 600 WP_003409989.1 L-lysine exporter -
FF956_RS10210 (FF956_10640) 2178341..2179252 + 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
FF956_RS10215 (FF956_10645) 2179337..2179567 - 231 WP_003409980.1 DUF167 domain-containing protein -
FF956_RS10220 (FF956_10650) 2179682..2180335 + 654 WP_003409976.1 cutinase Cfp21 -
FF956_RS10225 (FF956_10655) 2180323..2181972 - 1650 WP_003903703.1 PecA family PE domain-processing aspartic protease -
FF956_RS10230 (FF956_10660) 2182115..2182375 + 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FF956_RS10235 (FF956_10665) 2182384..2182803 + 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
FF956_RS10240 (FF956_10670) 2183028..2183996 + 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
FF956_RS10245 (FF956_10675) 2184187..2184873 + 687 WP_003409954.1 immunoprotective protein Mpt64 -
FF956_RS10250 (FF956_10680) 2185052..2186497 + 1446 WP_003409946.1 APC family permease -
FF956_RS10255 (FF956_10685) 2186460..2187308 - 849 WP_003409942.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T126764 WP_003409958.1 NZ_CP040692:2182384-2182803 [Mycobacterium tuberculosis complex sp. N0157]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T126764 NZ_CP040692:2182384-2182803 [Mycobacterium tuberculosis complex sp. N0157]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT126764 WP_003409968.1 NZ_CP040692:2182115-2182375 [Mycobacterium tuberculosis complex sp. N0157]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT126764 NZ_CP040692:2182115-2182375 [Mycobacterium tuberculosis complex sp. N0157]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References