Detailed information of TA system TA126763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2174606..2175504
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: FF956_RS10190
• Protein ID: WP_003410001.1
• Coordinates: 2174944..2175504 (+)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: FF956_RS10185
• Protein ID: WP_003410003.1
• Coordinates: 2174606..2174947 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS10150 (FF956_10575)	2170259..2170615	+	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
FF956_RS10155 (FF956_10580)	2170668..2170940	+	273	WP_003903706.1	DUF1490 family protein	-
FF956_RS10160 (FF956_10585)	2170937..2173252	+	2316	WP_003899120.1	cation transporter ATPase CptG	-
FF956_RS10165 (FF956_10590)	2173352..2173600	+	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
FF956_RS10170 (FF956_10595)	2173594..2173938	+	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
FF956_RS10175 (FF956_10600)	2174027..2174362	+	336	WP_003410009.1	dehydrogenase	-
FF956_RS10180 (FF956_10605)	2174263..2174520	-	258	WP_003410006.1	hypothetical protein	-
FF956_RS10185 (FF956_10610)	2174606..2174947	+	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
FF956_RS10190 (FF956_10615)	2174944..2175504	+	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
FF956_RS10195 (FF956_10625)	2176024..2176563	-	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
FF956_RS10200 (FF956_10630)	2176789..2177217	-	429	WP_003409992.1	cellulose-binding protein	-
FF956_RS10205 (FF956_10635)	2177633..2178232	-	600	WP_003409989.1	L-lysine exporter	-
FF956_RS10210 (FF956_10640)	2178341..2179252	+	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
FF956_RS10215 (FF956_10645)	2179337..2179567	-	231	WP_003409980.1	DUF167 domain-containing protein	-
FF956_RS10220 (FF956_10650)	2179682..2180335	+	654	WP_003409976.1	cutinase Cfp21	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T126763 WP_003410001.1 NZ_CP040692:2174944-2175504 [Mycobacterium tuberculosis complex sp. N0157]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T126763 NZ_CP040692:2174944-2175504 [Mycobacterium tuberculosis complex sp. N0157]
GTGAGCGATGCCCTCGATGAAGGGCTCGTCCAGCGTATCGACGCACGCGGAACAATTGAGTGGTCGGAAACGTGCTACCG
GTATACCGGCGCGCACCGTGACGCCTTGTCCGGTGAGGGCGCGCGCAGATTCGGAGGCAGGTGGAATCCGCCGCTGCTCT
TTCCGGCGATCTATCTTGCTGATTCCGCCCAAGCCTGCATGGTTGAGGTGGAACGGGCGGCGCAAGCGGCTTCAACGACC
GCAGAGAAGATGCTCGAGGCGGCCTACCGACTACACACGATCGACGTCACGGACCTGGCCGTCCTCGATCTGACAACCCC
GCAAGCTCGGGAAGCCGTGGGGCTCGAGAACGACGACATCTATGGCGACGACTGGTCAGGGTGCCAGGCGGTCGGACATG
CGGCCTGGTTCTTGCACATGCAAGGTGTCCTCGTGCCGGCGGCGGGCGGTGTCGGCCTCGTTGTCACCGCGTATGAACAG
CGAACTCGGCCGGGCCAACTACAACTGCGACAAAGCGTCGATCTGACGCCTGCTCTTTACCAAGAACTTCGAGCCACGTA
G

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT126763 WP_003410003.1 NZ_CP040692:2174606-2174947 [Mycobacterium tuberculosis complex sp. N0157]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT126763 NZ_CP040692:2174606-2174947 [Mycobacterium tuberculosis complex sp. N0157]
ATGGGAGTAAATGTGCTCGCCTCGACCGTGTCGGGTGCGATCGAGCGCTTGGGATTGACCTACGAGGAAGTCGGTGACAT
CGTCGATGCCTCGCCGCGTTCCGTGGCGCGATGGACCGCAGGTCAGGTGGTTCCCCAACGCCTCAACAAGCAACGACTTA
TCGAGCTGGCCTATGTCGCCGACGCCCTCGCGGAAGTGCTGCCGCGTGACCAGGCGAACGTGTGGATGTTTTCGCCGAAT
CGGTTACTGGAACACCGCAAGCCTGCCGACCTCGTGCGAGACGGCGAGTACCAACGCGTGTTGGCGCTCATCGACGCGAT
GGCGGAGGGAGTGTTCGTGTGA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4