Detailed information of TA system TA126761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2149572..2150198
• Replicon: chromosome
• Accession: NZ_CP040692
• Organism: Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FF956_RS10065
• Protein ID: WP_003410075.1
• Coordinates: 2149572..2149970 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FF956_RS10070
• Protein ID: WP_003911750.1
• Coordinates: 2149971..2150198 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF956_RS10035 (FF956_10455)	2145171..2146427	+	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
FF956_RS10040 (FF956_10460)	2146555..2147145	-	591	WP_003907840.1	IS110 family transposase	-
FF956_RS10045 (FF956_10465)	2147099..2147719	-	621	WP_003410086.1	IS110 family transposase	-
FF956_RS10050 (FF956_10470)	2147807..2147986	+	180	Protein_1982	hypothetical protein	-
FF956_RS10055 (FF956_10485)	2148423..2148857	-	435	WP_003914358.1	DUF1398 domain-containing protein	-
FF956_RS10060 (FF956_10490)	2148958..2149389	+	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FF956_RS10065 (FF956_10495)	2149572..2149970	-	399	WP_003410075.1	PIN domain nuclease	Toxin
FF956_RS10070 (FF956_10500)	2149971..2150198	-	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF956_RS10075 (FF956_10505)	2150457..2151626	+	1170	WP_003899126.1	ATP-binding protein	-
FF956_RS10080 (FF956_10510)	2151815..2152159	+	345	WP_003410065.1	ferredoxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T126761 WP_003410075.1 NZ_CP040692:c2149970-2149572 [Mycobacterium tuberculosis complex sp. N0157]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T126761 NZ_CP040692:c2149970-2149572 [Mycobacterium tuberculosis complex sp. N0157]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT126761 WP_003911750.1 NZ_CP040692:c2150198-2149971 [Mycobacterium tuberculosis complex sp. N0157]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT126761 NZ_CP040692:c2150198-2149971 [Mycobacterium tuberculosis complex sp. N0157]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8