Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PIN(toxin) |
| Location | 1544693..1545330 | Replicon | chromosome |
| Accession | NZ_CP040692 | ||
| Organism | Mycobacterium tuberculosis complex sp. N0157 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P0DMV7 |
| Locus tag | FF956_RS07190 | Protein ID | WP_003413180.1 |
| Coordinates | 1544935..1545330 (+) | Length | 132 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WJ44 |
| Locus tag | FF956_RS07185 | Protein ID | WP_003413183.1 |
| Coordinates | 1544693..1544938 (+) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| FF956_RS07165 (FF956_07475) | 1541287..1541799 | + | 513 | WP_003413197.1 | Holliday junction resolvase RuvX | - |
| FF956_RS07170 (FF956_07480) | 1541792..1543045 | + | 1254 | WP_003413196.1 | endolytic transglycosylase MltG | - |
| FF956_RS07175 (FF956_07485) | 1543042..1543851 | + | 810 | WP_003413193.1 | shikimate dehydrogenase | - |
| FF956_RS07180 (FF956_07490) | 1543863..1544282 | + | 420 | WP_003413190.1 | A24 family peptidase | - |
| FF956_RS07185 (FF956_07500) | 1544693..1544938 | + | 246 | WP_003413183.1 | type II toxin-antitoxin system antitoxin VapB20 | Antitoxin |
| FF956_RS07190 (FF956_07505) | 1544935..1545330 | + | 396 | WP_003413180.1 | type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 | Toxin |
| FF956_RS07195 (FF956_07510) | 1545430..1545804 | + | 375 | WP_003413177.1 | hypothetical protein | - |
| FF956_RS07200 (FF956_07515) | 1545820..1546197 | - | 378 | WP_003413174.1 | type II toxin-antitoxin system VapC family toxin | - |
| FF956_RS07205 (FF956_07520) | 1546194..1546451 | - | 258 | WP_003413167.1 | CopG family transcriptional regulator | - |
| FF956_RS07210 (FF956_07525) | 1546490..1546903 | - | 414 | WP_003413164.1 | PIN domain nuclease | - |
| FF956_RS07215 (FF956_07530) | 1546996..1547274 | - | 279 | WP_003901422.1 | type II toxin-antitoxin system VapB family antitoxin | - |
| FF956_RS07220 (FF956_07535) | 1547271..1547933 | - | 663 | WP_003903936.1 | LppA family lipoprotein | - |
| FF956_RS07225 (FF956_07540) | 1547930..1548589 | - | 660 | WP_003900846.1 | LppA family lipoprotein | - |
| FF956_RS07230 (FF956_07550) | 1548716..1549927 | - | 1212 | WP_003900845.1 | alpha/beta hydrolase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 132 a.a. Molecular weight: 14619.53 Da Isoelectric Point: 6.9794
>T126752 WP_003413180.1 NZ_CP040692:1544935-1545330 [Mycobacterium tuberculosis complex sp. N0157]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE
Download Length: 396 bp
>T126752 NZ_CP040692:1544935-1545330 [Mycobacterium tuberculosis complex sp. N0157]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG
Antitoxin
Download Length: 82 a.a. Molecular weight: 9098.33 Da Isoelectric Point: 5.6007
>AT126752 WP_003413183.1 NZ_CP040692:1544693-1544938 [Mycobacterium tuberculosis complex sp. N0157]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E
Download Length: 246 bp
>AT126752 NZ_CP040692:1544693-1544938 [Mycobacterium tuberculosis complex sp. N0157]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 5WZ4 | |
| PDB | 5WZF |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U4BW31 |