Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-PHD
Location 616657..617324 Replicon chromosome
Accession NZ_CP040692
Organism Mycobacterium tuberculosis complex sp. N0157

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag FF956_RS02835 Protein ID WP_003417916.1
Coordinates 616932..617324 (+) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8U8S7
Locus tag FF956_RS02830 Protein ID WP_003912214.1
Coordinates 616657..616932 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF956_RS02815 (FF956_02960) 612172..613044 + 873 WP_031708365.1 3-hydroxyacyl-thioester dehydratase HtdY -
FF956_RS02820 (FF956_02965) 613115..614914 - 1800 WP_031654355.1 PE family protein -
FF956_RS02825 (FF956_02970) 615104..616475 - 1372 Protein_556 ISNCY family transposase -
FF956_RS02830 (FF956_02975) 616657..616932 + 276 WP_003912214.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FF956_RS02835 (FF956_02980) 616932..617324 + 393 WP_003417916.1 type II toxin-antitoxin system VapC family toxin Toxin
FF956_RS02840 (FF956_02985) 618078..619130 + 1053 WP_003900678.1 polyprenyl synthetase family protein -
FF956_RS02845 (FF956_02990) 619130..620119 + 990 WP_003417912.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
FF956_RS02850 (FF956_02995) 620116..621954 + 1839 WP_019830554.1 1-deoxy-D-xylulose-5-phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 615104..615781 677


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T126739 WP_003417916.1 NZ_CP040692:616932-617324 [Mycobacterium tuberculosis complex sp. N0157]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T126739 NZ_CP040692:616932-617324 [Mycobacterium tuberculosis complex sp. N0157]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 9966.35 Da        Isoelectric Point: 4.5332

>AT126739 WP_003912214.1 NZ_CP040692:616657-616932 [Mycobacterium tuberculosis complex sp. N0157]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Download         Length: 276 bp

>AT126739 NZ_CP040692:616657-616932 [Mycobacterium tuberculosis complex sp. N0157]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BXS8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8U8S7

References