Detailed information of TA system TA126731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3165910..3166458
• Replicon: chromosome
• Accession: NZ_CP040691
• Organism: Mycobacterium tuberculosis complex sp. N0155

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: FF955_RS15100
• Protein ID: WP_003414602.1
• Coordinates: 3166195..3166458 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: FF955_RS15095
• Protein ID: WP_003414599.1
• Coordinates: 3165910..3166191 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF955_RS15070 (FF955_15600)	3161534..3162391	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
FF955_RS15075 (FF955_15605)	3162433..3163017	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
FF955_RS15080 (FF955_15610)	3163120..3163368	+	249	WP_003913411.1	antitoxin VapB23	-
FF955_RS15085 (FF955_15615)	3163365..3163745	+	381	WP_003414592.1	type II toxin-antitoxin system VapC family toxin	-
FF955_RS15090 (FF955_15620)	3163827..3165638	-	1812	WP_003414596.1	penicillin-binding protein	-
FF955_RS15095 (FF955_15625)	3165910..3166191	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
FF955_RS15100 (FF955_15630)	3166195..3166458	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FF955_RS15105 (FF955_15635)	3166831..3167685	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
FF955_RS15110 (FF955_15640)	3167741..3168904	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
FF955_RS15115 (FF955_15645)	3168921..3170135	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
FF955_RS15120 (FF955_15650)	3170143..3171384	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T126731 WP_003414602.1 NZ_CP040691:3166195-3166458 [Mycobacterium tuberculosis complex sp. N0155]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T126731 NZ_CP040691:3166195-3166458 [Mycobacterium tuberculosis complex sp. N0155]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT126731 WP_003414599.1 NZ_CP040691:3165910-3166191 [Mycobacterium tuberculosis complex sp. N0155]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT126731 NZ_CP040691:3165910-3166191 [Mycobacterium tuberculosis complex sp. N0155]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8