Detailed information of TA system TA126685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 693412..694058
• Replicon: chromosome
• Accession: NZ_CP040691
• Organism: Mycobacterium tuberculosis complex sp. N0155

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FF955_RS03115
• Protein ID: WP_003905350.1
• Coordinates: 693412..693804 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: FF955_RS03120
• Protein ID: WP_003403125.1
• Coordinates: 693801..694058 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF955_RS03100 (FF955_03150)	689074..690600	+	1527	WP_003905348.1	virulence factor Mce family protein	-
FF955_RS03105 (FF955_03155)	690663..691805	+	1143	WP_003911253.1	virulence factor Mce family protein	-
FF955_RS03110 (FF955_03160)	691810..693360	+	1551	WP_003905349.1	MCE family protein	-
FF955_RS03115 (FF955_03165)	693412..693804	-	393	WP_003905350.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
FF955_RS03120 (FF955_03170)	693801..694058	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
FF955_RS03125 (FF955_03175)	694241..695476	-	1236	WP_003403128.1	ATP-binding protein	-
FF955_RS03130 (FF955_03180)	695727..696140	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
FF955_RS03135 (FF955_03185)	696137..696373	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	-
FF955_RS03140 (FF955_03190)	696477..696983	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FF955_RS03145 (FF955_03195)	697097..697627	-	531	WP_003917320.1	two component system sensor kinase HK2	-
FF955_RS03150 (FF955_03200)	697611..698306	-	696	WP_003918099.1	two-component system response regulator TcrA	-
FF955_RS03155 (FF955_03205)	698429..698740	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14094.11 Da
• Isoelectric Point: 4.6558

>T126685 WP_003905350.1 NZ_CP040691:c693804-693412 [Mycobacterium tuberculosis complex sp. N0155]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDIEVITI

Nucleotide

• Length: 393 bp

>T126685 NZ_CP040691:c693804-693412 [Mycobacterium tuberculosis complex sp. N0155]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACATCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT126685 WP_003403125.1 NZ_CP040691:c694058-693801 [Mycobacterium tuberculosis complex sp. N0155]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT126685 NZ_CP040691:c694058-693801 [Mycobacterium tuberculosis complex sp. N0155]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Antitoxin

• AlphaFold DB: G0TQ86