Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3125697..3126301 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag FF954_RS14845 Protein ID WP_003414492.1
Coordinates 3125697..3126089 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag FF954_RS14850 Protein ID WP_003414495.1
Coordinates 3126086..3126301 (-) Length 72 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS14815 (FF954_15330) 3120847..3121635 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
FF954_RS14820 (FF954_15335) 3121969..3122514 - 546 WP_003904931.1 DUF1802 family protein -
FF954_RS14825 (FF954_15340) 3122786..3123670 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
FF954_RS14830 (FF954_15345) 3123673..3124560 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
FF954_RS14835 (FF954_15350) 3124865..3125410 - 546 WP_003908074.1 DUF1802 family protein -
FF954_RS14840 (FF954_15355) 3125407..3125676 - 270 WP_003414489.1 DUF2277 family protein -
FF954_RS14845 (FF954_15360) 3125697..3126089 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
FF954_RS14850 (FF954_15365) 3126086..3126301 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FF954_RS14855 (FF954_15370) 3126348..3127058 + 711 WP_031663819.1 enoyl-CoA hydratase -
FF954_RS14860 (FF954_15375) 3127137..3128219 - 1083 Protein_2933 ABC transporter ATP-binding protein -
FF954_RS14865 (FF954_15380) 3128212..3129522 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
FF954_RS14870 (FF954_15385) 3129525..3130352 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
FF954_RS14875 (FF954_15390) 3130349..3131260 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T126670 WP_003414492.1 NZ_CP040690:c3126089-3125697 [Mycobacterium tuberculosis complex sp. N0153]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T126670 NZ_CP040690:c3126089-3125697 [Mycobacterium tuberculosis complex sp. N0153]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT126670 WP_003414495.1 NZ_CP040690:c3126301-3126086 [Mycobacterium tuberculosis complex sp. N0153]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT126670 NZ_CP040690:c3126301-3126086 [Mycobacterium tuberculosis complex sp. N0153]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8


Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References