Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3061588..3062275 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag FF954_RS14485 Protein ID WP_003414064.1
Coordinates 3061880..3062275 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag FF954_RS14480 Protein ID WP_003414061.1
Coordinates 3061588..3061854 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS14455 (FF954_14975) 3057227..3058129 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
FF954_RS14460 (FF954_14980) 3058198..3058950 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
FF954_RS14465 (FF954_14985) 3059194..3059469 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
FF954_RS14470 (FF954_14990) 3059466..3061088 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
FF954_RS14475 (FF954_14995) 3061175..3061591 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
FF954_RS14480 (FF954_15000) 3061588..3061854 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
FF954_RS14485 (FF954_15005) 3061880..3062275 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
FF954_RS14490 (FF954_15010) 3062272..3062541 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
FF954_RS14495 (FF954_15015) 3062551..3063645 - 1095 WP_003414068.1 restriction endonuclease subunit S -
FF954_RS14500 (FF954_15020) 3063642..3064061 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
FF954_RS14505 3064060..3064134 + 75 Protein_2862 hypothetical protein -
FF954_RS14510 (FF954_15025) 3064135..3064614 - 480 WP_003414073.1 dihydrofolate reductase -
FF954_RS14515 (FF954_15030) 3064685..3065485 - 801 WP_003911953.1 thymidylate synthase -
FF954_RS14520 (FF954_15035) 3065641..3066378 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T126667 WP_003414064.1 NZ_CP040690:c3062275-3061880 [Mycobacterium tuberculosis complex sp. N0153]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T126667 NZ_CP040690:c3062275-3061880 [Mycobacterium tuberculosis complex sp. N0153]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT126667 WP_003414061.1 NZ_CP040690:c3061854-3061588 [Mycobacterium tuberculosis complex sp. N0153]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT126667 NZ_CP040690:c3061854-3061588 [Mycobacterium tuberculosis complex sp. N0153]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TF65


Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References