Detailed information of TA system TA126661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2855529..2856169
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: FF954_RS13350
• Protein ID: WP_003412970.1
• Coordinates: 2855529..2855948 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: FF954_RS13355
• Protein ID: WP_003412975.1
• Coordinates: 2855945..2856169 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS13320 (FF954_13795)	2851114..2851836	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
FF954_RS13325 (FF954_13805)	2852353..2852580	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
FF954_RS13330 (FF954_13810)	2852577..2852978	+	402	WP_003412963.1	PIN domain-containing protein	-
FF954_RS13335 (FF954_13815)	2853013..2853933	-	921	WP_003412965.1	restriction endonuclease	-
FF954_RS13340	2854274..2854519	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
FF954_RS13345 (FF954_13820)	2854578..2855528	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
FF954_RS13350 (FF954_13825)	2855529..2855948	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
FF954_RS13355 (FF954_13830)	2855945..2856169	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
FF954_RS13360 (FF954_13835)	2856200..2859043	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
FF954_RS13365 (FF954_13840)	2859115..2859516	-	402	WP_003412981.1	hypothetical protein	-
FF954_RS13370 (FF954_13845)	2859516..2859986	-	471	WP_003412985.1	transcription antitermination factor NusB	-
FF954_RS13375 (FF954_13850)	2859989..2860552	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T126661 WP_003412970.1 NZ_CP040690:c2855948-2855529 [Mycobacterium tuberculosis complex sp. N0153]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T126661 NZ_CP040690:c2855948-2855529 [Mycobacterium tuberculosis complex sp. N0153]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT126661 WP_003412975.1 NZ_CP040690:c2856169-2855945 [Mycobacterium tuberculosis complex sp. N0153]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT126661 NZ_CP040690:c2856169-2855945 [Mycobacterium tuberculosis complex sp. N0153]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09