Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2324394..2325030 Replicon chromosome
Accession NZ_CP040690
Organism Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag FF954_RS10835 Protein ID WP_003904769.1
Coordinates 2324620..2325030 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P9WJ84
Locus tag FF954_RS10830 Protein ID WP_003410651.1
Coordinates 2324394..2324627 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FF954_RS10815 2319851..2320243 + 393 WP_229303073.1 metal ABC transporter permease -
FF954_RS10820 (FF954_11200) 2320244..2320648 - 405 WP_003410645.1 PPOX class F420-dependent oxidoreductase -
FF954_RS10825 (FF954_11205) 2320732..2324316 - 3585 WP_003410648.1 cobaltochelatase subunit CobN -
FF954_RS10830 (FF954_11210) 2324394..2324627 + 234 WP_003410651.1 type II toxin-antitoxin system antitoxin MazE7 Antitoxin
FF954_RS10835 (FF954_11215) 2324620..2325030 + 411 WP_003904769.1 type II toxin-antitoxin system toxin endoribonuclease MazF7 Toxin
FF954_RS10840 (FF954_11220) 2325014..2326220 + 1207 Protein_2143 precorrin-3B synthase -
FF954_RS10845 (FF954_11225) 2326230..2326856 + 627 WP_003410658.1 precorrin-8X methylmutase -
FF954_RS10850 (FF954_11230) 2326853..2328379 + 1527 WP_003410659.1 precorrin-2 C(20)-methyltransferase -
FF954_RS10855 (FF954_11235) 2328325..2329548 - 1224 WP_003901321.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14176.35 Da        Isoelectric Point: 9.4835

>T126657 WP_003904769.1 NZ_CP040690:2324620-2325030 [Mycobacterium tuberculosis complex sp. N0153]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRGV
RAVARARLVERLGALKPATMPAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Download         Length: 411 bp

>T126657 NZ_CP040690:2324620-2325030 [Mycobacterium tuberculosis complex sp. N0153]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCCCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8450.25 Da        Isoelectric Point: 4.7954

>AT126657 WP_003410651.1 NZ_CP040690:2324394-2324627 [Mycobacterium tuberculosis complex sp. N0153]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Download         Length: 234 bp

>AT126657 NZ_CP040690:2324394-2324627 [Mycobacterium tuberculosis complex sp. N0153]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7DU4
PDB 6A6X
AlphaFold DB A0A7U4BV50

References