Detailed information of TA system TA126655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2256608..2257234
• Replicon: chromosome
• Accession: NZ_CP040690
• Organism: Mycobacterium tuberculosis complex sp. N0153

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: FF954_RS10545
• Protein ID: WP_003410075.1
• Coordinates: 2256836..2257234 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: FF954_RS10540
• Protein ID: WP_003911750.1
• Coordinates: 2256608..2256835 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FF954_RS10530 (FF954_10900)	2254647..2254991	-	345	WP_003410065.1	ferredoxin family protein	-
FF954_RS10535 (FF954_10905)	2255180..2256349	-	1170	WP_003899126.1	ATP-binding protein	-
FF954_RS10540 (FF954_10910)	2256608..2256835	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
FF954_RS10545 (FF954_10915)	2256836..2257234	+	399	WP_003410075.1	PIN domain nuclease	Toxin
FF954_RS10550 (FF954_10920)	2257417..2257848	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
FF954_RS10555 (FF954_10925)	2257997..2258383	+	387	WP_003899128.1	DUF1398 domain-containing protein	-
FF954_RS10560 (FF954_10940)	2258820..2258999	-	180	Protein_2087	hypothetical protein	-
FF954_RS10565 (FF954_10945)	2259087..2259707	+	621	WP_003410086.1	IS110 family transposase	-
FF954_RS10570 (FF954_10950)	2259661..2260251	+	591	WP_003899131.1	IS110 family transposase	-
FF954_RS10575 (FF954_10955)	2260379..2261635	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T126655 WP_003410075.1 NZ_CP040690:2256836-2257234 [Mycobacterium tuberculosis complex sp. N0153]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T126655 NZ_CP040690:2256836-2257234 [Mycobacterium tuberculosis complex sp. N0153]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT126655 WP_003911750.1 NZ_CP040690:2256608-2256835 [Mycobacterium tuberculosis complex sp. N0153]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT126655 NZ_CP040690:2256608-2256835 [Mycobacterium tuberculosis complex sp. N0153]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8